Application

This Anti-14-3-3 ε Antibody, N-terminal is validated for use in WB, IH for the detection of 14-3-3 ε.

Western blot: 1:3,000 using ECL. Immunohistochemistry: 1:200-1:400 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes. ELISA Optimal working dilutions must be determined by end user.

General description

Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s. r> r> The 14-3-3 epsilon, C-terminal antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.

Immunogen

Synthetic peptide from the N-terminal area of sheep 14-3-3 epsilon.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

14-3-3 epsilon, N-terminal. By Western blot the antibody recognizes the ~33 kDa protein.